Freesia plantlets from flower-buds cultivated in vitro.

Supercooling in overwintering azalea flower buds.

Differential thermal analysis and nuclear magnetic resonance spectroscopy experiments on whole flower buds and excised floral primordia of azalea (Rhododendron kosterianum, Schneid.) proved that supercooling is the mode of freezing resistance (avoidance) of azalea flower primordia. Increase in the linewidth of nuclear magnetic resonance spectra for water upon thawing supports the view that inju...

Protecting Stone Fruit Flower Buds from Winter Freeze Damage

Flower buds of peach, apricot, and sweet cherry are killed by low temperatures during winter and spring. Frost protection measures used commonly in the spring are applied to freeze protection during the winter in the Yakima Valley of Washington. Critical temperatures change rapidly during winter. To succeed, winter freeze protection requires adequate inversions, equipment that operates at tempe...

In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.

In vitro flowering and effective micropropagation protocol were studied in Swertia chirayita, an important medicinal plant using axillary bud explants. The Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) 1.0 mg L(-1) and adenine sulfate 70.0 mg L(-1) was found optimum for production of multiple shoots. In the present study, incubation of flowering cultures on BAP s...

Live confocal imaging of Arabidopsis flower buds.

Recent advances in confocal microscopy, coupled with the development of numerous fluorescent reporters, provide us with a powerful tool to study the development of plants. Live confocal imaging has been used extensively to further our understanding of the mechanisms underlying the formation of roots, shoots and leaves. However, it has not been widely applied to flowers, partly because of specif...

The growth, development and phosphatase activity of embryonic avian femora and limb-buds cultivated in vitro.